tsa-201 cells (hek-293 cell line stably expressing sv40 temperaturesensitive t-antigen (European Collection of Authenticated Cell Cultures)

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European Collection of Authenticated Cell Cultures tsa-201 cells (hek-293 cell line stably expressing sv40 temperaturesensitive t-antigen
Tsa 201 Cells (Hek 293 Cell Line Stably Expressing Sv40 Temperaturesensitive T Antigen, supplied by European Collection of Authenticated Cell Cultures, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/tsa-201+cells/tsa+201+cells/pm39370994-67-0-14
Average 90 stars, based on 1 article reviews

tsa-201 cells (hek-293 cell line stably expressing sv40 temperaturesensitive t-antigen - by Bioz Stars, 2026-10

90/100 stars

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Cell Culture:

Article Title: Activity-dependent regulation of T-type calcium channels by submembrane calcium ions
Article Snippet: tsA-201 cells (RRID: CVCL_2737 ) were obtained from the European Collection of Authenticated Cell Cultures (ECACC 96121229).

Article Title: β2-subunit alternative splicing stabilizes Cav2.3 Ca 2+ channel activity during continuous midbrain dopamine neuron-like activity
Article Snippet: Cell line ( Homo sapiens ) , TsA-201 cells , European Collection of Authenticated Cell Cultures , ECACC, catalogue # 96121229, lot # 13D034 , Tested Mycoplasma negative.

Article Title: Xiaoyu Jiangzhi capsule protects against heart failure via Ca2+/CaMKII signaling pathways in mice
Article Snippet: Human tsA-201 cells (ECACC, European Collection of Authenticated Cell Cultures, London, UK; catalog No. CBP61213), a simian virus 40 T-antigen expressing derivative of the human embryonic kidney cell line HEK293, were maintained in monolayer culture in Dulbecco's modified Eagle's medium (DMEM; Gibco,Waltham,MA) enriched with 10% fetal bovine serum (Gibco, Waltham, MA), 100 U/mL penicillin, and 100 ug/mL streptomycin.

Article Title: A Gs-coupled purinergic receptor boosts Ca2+ influx and vascular contractility during diabetic hyperglycemia
Article Snippet: These cells are included in the European Collection of Authenticated Cell Cultures. tsA-201 cells were cultured in DMEM supple- mented with 1X pyruvate, 1X glutamax, 8% fetal bovine serum (FBS) and 5 mM glucose (without phenol red) at 37 ̊ C in a 5% CO2 incubator.

Article Title: The Ca V β Subunit Protects the I-II Loop of the Voltage-gated Calcium Channel Ca V 2.2 from Proteasomal Degradation but Not Oligoubiquitination
Article Snippet: tsA-201 cells (European Collection of Authenticated Cell Cultures (ECACC)) were cultured in Dulbecco's modified Eagle's medium in the presence of 10% fetal bovine serum, penicillin, streptomycin, and 2% GlutaMAX (Invitrogen).

Article Title: A G s -coupled purinergic receptor boosts Ca 2+ influx and vascular contractility during diabetic hyperglycemia
Article Snippet: These cells are included in the European Collection of Authenticated Cell Cultures. tsA-201 cells were cultured in DMEM supplemented with 1X pyruvate, 1X glutamax, 8% fetal bovine serum (FBS) and 5 mM glucose (without phenol red) at 37° C in a 5% CO 2 incubator.

Article Title: LRP1 influences trafficking of N-type calcium channels via interaction with the auxiliary α 2 δ-1 subunit
Article Snippet: The tsA-201 cells (European Collection of Authenticated Cell Cultures), tested to be mycoplasma-free, were cultured in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% foetal bovine serum (FBS), 1 unit/ml penicillin, 1 μg/ml streptomycin and 1% GlutaMAX (Life Technologies, Waltham, MA).

Article Title: Germline de novo variant F747S extends the phenotypic spectrum of CACNA1D Ca2+ channelopathies.
Article Snippet: TsA-201 cells were obtained from the European Collection of Authenticated Cell Cultures (ECACC, catalogue number 96121229, lot number 13D034) at passage 6.

Modification:

Article Title: A G s -coupled purinergic receptor boosts Ca 2+ influx and vascular contractility during diabetic hyperglycemia
Article Snippet: These cells are included in the European Collection of Authenticated Cell Cultures. tsA-201 cells were cultured in DMEM supplemented with 1X pyruvate, 1X glutamax, 8% fetal bovine serum (FBS) and 5 mM glucose (without phenol red) at 37° C in a 5% CO 2 incubator.

Patch Clamp:

Article Title: A G s -coupled purinergic receptor boosts Ca 2+ influx and vascular contractility during diabetic hyperglycemia
Article Snippet: These cells are included in the European Collection of Authenticated Cell Cultures. tsA-201 cells were cultured in DMEM supplemented with 1X pyruvate, 1X glutamax, 8% fetal bovine serum (FBS) and 5 mM glucose (without phenol red) at 37° C in a 5% CO 2 incubator.

Activation Assay:

Article Title: A G s -coupled purinergic receptor boosts Ca 2+ influx and vascular contractility during diabetic hyperglycemia
Article Snippet: These cells are included in the European Collection of Authenticated Cell Cultures. tsA-201 cells were cultured in DMEM supplemented with 1X pyruvate, 1X glutamax, 8% fetal bovine serum (FBS) and 5 mM glucose (without phenol red) at 37° C in a 5% CO 2 incubator.

Transfection:

Article Title: A G s -coupled purinergic receptor boosts Ca 2+ influx and vascular contractility during diabetic hyperglycemia
Article Snippet: These cells are included in the European Collection of Authenticated Cell Cultures. tsA-201 cells were cultured in DMEM supplemented with 1X pyruvate, 1X glutamax, 8% fetal bovine serum (FBS) and 5 mM glucose (without phenol red) at 37° C in a 5% CO 2 incubator.

Construct:

Article Title: A G s -coupled purinergic receptor boosts Ca 2+ influx and vascular contractility during diabetic hyperglycemia
Article Snippet: These cells are included in the European Collection of Authenticated Cell Cultures. tsA-201 cells were cultured in DMEM supplemented with 1X pyruvate, 1X glutamax, 8% fetal bovine serum (FBS) and 5 mM glucose (without phenol red) at 37° C in a 5% CO 2 incubator.

Variant Assay:

Article Title: A G s -coupled purinergic receptor boosts Ca 2+ influx and vascular contractility during diabetic hyperglycemia
Article Snippet: These cells are included in the European Collection of Authenticated Cell Cultures. tsA-201 cells were cultured in DMEM supplemented with 1X pyruvate, 1X glutamax, 8% fetal bovine serum (FBS) and 5 mM glucose (without phenol red) at 37° C in a 5% CO 2 incubator.

Clone Assay:

Article Title: A G s -coupled purinergic receptor boosts Ca 2+ influx and vascular contractility during diabetic hyperglycemia
Article Snippet: These cells are included in the European Collection of Authenticated Cell Cultures. tsA-201 cells were cultured in DMEM supplemented with 1X pyruvate, 1X glutamax, 8% fetal bovine serum (FBS) and 5 mM glucose (without phenol red) at 37° C in a 5% CO 2 incubator.

Plasmid Preparation:

Article Title: A G s -coupled purinergic receptor boosts Ca 2+ influx and vascular contractility during diabetic hyperglycemia
Article Snippet: These cells are included in the European Collection of Authenticated Cell Cultures. tsA-201 cells were cultured in DMEM supplemented with 1X pyruvate, 1X glutamax, 8% fetal bovine serum (FBS) and 5 mM glucose (without phenol red) at 37° C in a 5% CO 2 incubator.

Lysis:

Article Title: A G s -coupled purinergic receptor boosts Ca 2+ influx and vascular contractility during diabetic hyperglycemia
Article Snippet: These cells are included in the European Collection of Authenticated Cell Cultures. tsA-201 cells were cultured in DMEM supplemented with 1X pyruvate, 1X glutamax, 8% fetal bovine serum (FBS) and 5 mM glucose (without phenol red) at 37° C in a 5% CO 2 incubator.

cDNA Synthesis:

Article Title: A G s -coupled purinergic receptor boosts Ca 2+ influx and vascular contractility during diabetic hyperglycemia
Article Snippet: These cells are included in the European Collection of Authenticated Cell Cultures. tsA-201 cells were cultured in DMEM supplemented with 1X pyruvate, 1X glutamax, 8% fetal bovine serum (FBS) and 5 mM glucose (without phenol red) at 37° C in a 5% CO 2 incubator.

Picogreen Assay:

Article Title: A G s -coupled purinergic receptor boosts Ca 2+ influx and vascular contractility during diabetic hyperglycemia
Article Snippet: These cells are included in the European Collection of Authenticated Cell Cultures. tsA-201 cells were cultured in DMEM supplemented with 1X pyruvate, 1X glutamax, 8% fetal bovine serum (FBS) and 5 mM glucose (without phenol red) at 37° C in a 5% CO 2 incubator.

RNAscope:

Article Title: A G s -coupled purinergic receptor boosts Ca 2+ influx and vascular contractility during diabetic hyperglycemia
Article Snippet: These cells are included in the European Collection of Authenticated Cell Cultures. tsA-201 cells were cultured in DMEM supplemented with 1X pyruvate, 1X glutamax, 8% fetal bovine serum (FBS) and 5 mM glucose (without phenol red) at 37° C in a 5% CO 2 incubator.

Multiplex Assay:

Article Title: A G s -coupled purinergic receptor boosts Ca 2+ influx and vascular contractility during diabetic hyperglycemia
Article Snippet: These cells are included in the European Collection of Authenticated Cell Cultures. tsA-201 cells were cultured in DMEM supplemented with 1X pyruvate, 1X glutamax, 8% fetal bovine serum (FBS) and 5 mM glucose (without phenol red) at 37° C in a 5% CO 2 incubator.

Software:

Article Title: A G s -coupled purinergic receptor boosts Ca 2+ influx and vascular contractility during diabetic hyperglycemia
Article Snippet: These cells are included in the European Collection of Authenticated Cell Cultures. tsA-201 cells were cultured in DMEM supplemented with 1X pyruvate, 1X glutamax, 8% fetal bovine serum (FBS) and 5 mM glucose (without phenol red) at 37° C in a 5% CO 2 incubator.

Virus:

Article Title: A G s -coupled purinergic receptor boosts Ca 2+ influx and vascular contractility during diabetic hyperglycemia
Article Snippet: These cells are included in the European Collection of Authenticated Cell Cultures. tsA-201 cells were cultured in DMEM supplemented with 1X pyruvate, 1X glutamax, 8% fetal bovine serum (FBS) and 5 mM glucose (without phenol red) at 37° C in a 5% CO 2 incubator.

Expressing:

Article Title: A G s -coupled purinergic receptor boosts Ca 2+ influx and vascular contractility during diabetic hyperglycemia
Article Snippet: These cells are included in the European Collection of Authenticated Cell Cultures. tsA-201 cells were cultured in DMEM supplemented with 1X pyruvate, 1X glutamax, 8% fetal bovine serum (FBS) and 5 mM glucose (without phenol red) at 37° C in a 5% CO 2 incubator.

Incubation:

Article Title: A G s -coupled purinergic receptor boosts Ca 2+ influx and vascular contractility during diabetic hyperglycemia
Article Snippet: These cells are included in the European Collection of Authenticated Cell Cultures. tsA-201 cells were cultured in DMEM supplemented with 1X pyruvate, 1X glutamax, 8% fetal bovine serum (FBS) and 5 mM glucose (without phenol red) at 37° C in a 5% CO 2 incubator.

Saline:

Article Title: A G s -coupled purinergic receptor boosts Ca 2+ influx and vascular contractility during diabetic hyperglycemia
Article Snippet: These cells are included in the European Collection of Authenticated Cell Cultures. tsA-201 cells were cultured in DMEM supplemented with 1X pyruvate, 1X glutamax, 8% fetal bovine serum (FBS) and 5 mM glucose (without phenol red) at 37° C in a 5% CO 2 incubator.

Review

tsa-201 cells (hek-293 cell line stably expressing sv40 temperaturesensitive t-antigen (European Collection of Authenticated Cell Cultures)

Structured Review

Images

Related Articles

Cell Culture:

Modification:

Patch Clamp:

Activation Assay:

Transfection:

Construct:

Variant Assay:

Clone Assay:

Plasmid Preparation:

Lysis:

cDNA Synthesis:

Picogreen Assay:

RNAscope:

Multiplex Assay:

Software:

Virus:

Expressing:

Incubation:

Saline:

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